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- Title
Comparative Study of Regulatory T Cell Function of Human CD25<sup>+</sup>CD4<sup>+</sup> T Cells from Thymocytes, Cord Blood, and Adult Peripheral Blood.
- Authors
Fujimaki, Wakae; Takahashi, Nozomu; Ohnuma, Kei; Nagatsu, Masayoshi; Kurosawa, Hiromi; Yoshida, Satoko; Dang, Nam H.; Uchiyama, Takehiko; Morimoto, Chikao
- Abstract
CD25+CD4+ regulatory T cells suppress T cell activation and regulatemultiple immune reactions in in vitro and in vivo studies. To define the regulatory function of human CD25+CD4+ T cells at various stages of maturity, we investigated in detail the functional differences of CD25+CD4+ T cells from thymocytes, cord blood (CB), and adult peripheral blood (APB). CB CD25+CD4+ T cells displayed low-FOXP3 protein expression level and had no suppressive activity. In contrast, CD25+CD4+ T cells from thymocytes or APB expressed high expression level of FOXP3 protein associated with significant suppressive activity. Although CB CD25+CD4+ T cells exhibited no suppressive activity, striking suppressive activity was observed following expansion in culture associated with increased FOXP3 expression and a shift from the CD45RA+ to the CD45RA- phenotype. These functional differences in CD25+CD4+ T cells from Thy, CB, and APB hence suggest a pathway of maturation for Treg in the peripheral immune system.
- Subjects
MEDICAL research; T cells; LYMPHOCYTES; IMMUNE system; COMPARATIVE studies
- Publication
Clinical & Developmental Immunology, 2008, p1
- ISSN
1740-2522
- Publication type
Article
- DOI
10.1155/2008/305859