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- Title
Diallyl disulfide regulates proliferation, apoptosis and aerobic glycolysis by down-regulating PKM2 in gastric cancer cells.
- Authors
Zhiyan LI; Baiwei ZHAO; Deyao ZHANG; Juan DENG; Yongming CHEN; Jian ZHOU
- Abstract
BACKGROUND: The aim of this study was to identify the mechanisms by which diallyl disulfide (DADS) regulates proliferation, apoptosis and aerobic glycolysis by down-regulating PKM2 in gastric cancer cells. METHODS: HGC-27 gastric cancer cells were cultured. The glucose concentration and lactate level in the medium were examined using a Glucose Colorimetric Assay Kit II and a Lactate Assay Kit, respectively. Cell lysates were analyzed by western blot using antibodies specific for PKM2, LDHA, Mcl-1, Bcl-XL and GAPDH, and the mRNA levels of PKM2 were determined by real-time PCR using specific primers. shRNAPKM2 was transfected into HGC-27 cells using Lipofectamine 2000. Cellular growth inhibition was assayed using the CCK8 method. Statistical analyses were performed using SPSS 17.0 software. RESULTS: Inhibition of anaerobic glycolysis was observed in diallyl disulfide-treated HGC-27 cells, including an increased glucose concentration in the medium, decreased lactate production in the medium and down-regulation of PKM2, LDHA, Mcl-1 and Bcl-xl expression. Furthermore, down-regulation of PKM2 inhibited proliferation and induced apoptosis in HGC-27 human gastric cancer cells in vitro and in vivo. We further found that DADS did not significantly suppress anaerobic glycolysis or proliferation and induced apoptosis when down-regulating PKM2. CONCLUSIONS: DADS suppressed proliferation and induced apoptosis in HGC-27 human gastric cancer cells by suppressing aerobic glycolysis through the down-regulation of PKM2. These results indicated that PKM2 may serve as a potential gene therapy target.
- Subjects
DIALLYL disulfide; APOPTOSIS; GLYCOLYSIS; PYRUVATE kinase; STOMACH cancer
- Publication
Minerva Biotechnology & Biomolecular Research, 2021, Vol 33, Issue 3, p135
- ISSN
2724-542X
- Publication type
Article
- DOI
10.23736/S2724-542X.20.02634-8