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- Title
Differential response of human naive and memory/effector T cells to dendritic cells infected by respiratory syncytial virus.
- Authors
Rothoeft, T.; Fischer, K.; Zawatzki, S.; Schulz, V.; Schauer, U.; Rettberg, C. Körner
- Abstract
In vitro studies have contributed substantially to the understanding of immunopathology of respiratory syncytial virus (RSV)-mediated disease. In the present study we compared the effect of RSV-infected dendritic cells on the time–course of the primary and memory/effector T cell response in vitro. Cultures with uninfected dendritic cells known to elicit T helper 2 (Th2) responses and with polyinosinic-polycytidylic acid (poly-IC)-stimulated dendritic cells known to elicit Th1 responses served as controls. At day 1 after stimulation there was a high proportion of interleukin (IL)-2 and tumour necrosis factor (TNF)-α-producing T cells with no difference in number of producing T cells as well as concentration of secreted cytokines between RSV-infected and control cultures. However, up to day 3 generation of IFN-γ was reduced markedly. In addition, there was a reduced proliferation in RSV cultures. At day 7 the RSV-treated cultures showed a preponderance of IL-4 generation. At days 21–24, after three rounds of restimulation, memory/effector T cells matured under the influence of RSV were still not fully polarized but in contrast to the primary response displayed a predominance of Th1 cytokines. Contact with RSV-infected HEp-2 cells inhibited proliferation of T cells; memory effector T cells were less sensitive to contact inhibition than naive T cells. In addition, RSV inhibited the stimulated rearrangement of cortical actin more effectively in naive compared to memory T cells. In summary, we have shown that RSV infection of dendritic cells has a distinct modulatory effect on the primary response and a less pronounced effect on the memory response.
- Subjects
ANTIGEN presenting cells; DENDRITIC cells; T cells; CYTOKINES; RESPIRATORY syncytial virus
- Publication
Clinical & Experimental Immunology, 2007, Vol 150, Issue 2, p263
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/j.1365-2249.2007.03497.x