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- Title
Detection of HLA class II-dependent T helper antigen using antigen phage display.
- Authors
Somasondaram, R.; Satyamoorthy, K.; Caputo, L.; Yssel, H.; Herlyn, D.
- Abstract
Major histocompatibility complex (MHC) class II-dependent antigens not only activate CD4+ T helper (Th) cells, but also cytolytic T lymphocytes and effector cells of the innate immune system. These antigens therefore are candidate vaccines against cancer and infectious agents. We have developed a novel approach using a model antigen, tetanus toxoid (TT), which provides the basis for the establishment of a novel strategy of cloning Th antigens. In the TT model system, a cDNA library encoding part of the TT light chain which contained a TT-associated Th epitope recognized by TT-specific Th clones was displayed on a phage vector (TT-phage) and presented to TT-specific Th cells by autologous Epstein-Barr virus-transformed B cells (APC). These TT-phages were able to specifically activate two different TT-specific CD4+ Th cell lines as demonstrated both in [3H]thymidine incorporation and cytokine release assays. Th cell stimulation by TT-phages was significant at a ratio of one TT-phage in 50 irrelevant phages. The described approach provides the basis for the development of a novel strategy of cloning MHC class II-dependent Th antigens, using available Th cells. This strategy has several potential advantages over existing antigen cloning methods or biochemical peptide isolation.
- Subjects
MAJOR histocompatibility complex; ANTIGENS; CLONING; ANAEROBIC infections; TETANUS; IMMUNE system; EPSTEIN-Barr virus; CANCER treatment; DNA vaccines; CD4 antigen
- Publication
Clinical & Experimental Immunology, 2004, Vol 135, Issue 2, p247
- ISSN
0009-9104
- Publication type
Article
- DOI
10.1111/j.1365-2249.2003.02366.x