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- Title
Detection of Porcine Circovirus Type 2 (PCV2) in Mosquitoes from Pig Farms by PCR.
- Authors
Xiaohong Yang; Lidan Hou; Jing Ye; Qigai He; Shengbo Cao
- Abstract
To investigate, whether mosquitoes could be potential vectors that maintain or transmit PCV2, 59 mosquito samples were collected from suspicious PCV2-infected pig farms in Hubei and Anhui province, China. Total DNA from these mosquitoes was extracted and then tested for presence of PCV2 nucleic acid by PCR. Four (6.78%) samples showed the positive result. Subsequently, the positive PCR product was cloned into pEASY-T1 vector and sequenced. Sequence analysis displayed that homology between the PCR products and PCV2 strains were more than 94%. These results demonstrate that PCV2 nucleic acid exists in these mosquitoes, which suggests that mosquitoes could serve as mechanical transmission vectors of PCV2.
- Subjects
ANHUI Sheng (China); CHINA; CIRCOVIRUSES; POLYMERASE chain reaction; SWINE farms; NUCLEIC acids; MOSQUITO vectors
- Publication
Pakistan Veterinary Journal, 2012, Vol 32, Issue 1, p134
- ISSN
0253-8318
- Publication type
Article