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- Title
Evaluation of recombinant sporozoite antigen SPAG-1 as a vaccine candidate against Theileria annulata by the use of different delivery systems.
- Authors
Boulter, Nicola; Brown, Duncan; Wilkie, Gwen; Williamson, Susanna; Kirvar, Erol; Knight, Pamela; Glass, Elizabeth; Campbell, John; Morzaria, Subhash; Nene, Vish; Musoke, Anthony; d'oliveira, Christine; Gubbels, Marc-Jan; Jongejan, Frans; Hall, Roger; Boulter, N; Brown, D; Wilkie, G; Williamson, S; Kirvar, E
- Abstract
The major sporozoite surface antigen of Theileria annulata (SPAG-1) is a candidate for inclusion in a subunit vaccine. In this paper we summarize the results of 4 vaccination experiments using recombinant SPAG-1 expressed in different systems and presented in different adjuvants. The antigen has been presented as either a C terminal 108 amino acid peptide (called SR1) expressed as both beta-galactosidase and hepatitis B core antigen fusions or as a full-length form expressed as a GST fusion with an N terminal His6 tag. We used different adjuvants, namely Freund's, saponin, ISCOMs and a proprietary adjuvant supplied by SmithKline Beecham, which we call SKBA. The data point to the conclusion that SPAG-1 can elicit partial protection and is therefore suitable for inclusion in an eventual multicomponent subunit vaccine.
- Subjects
CELL surface antigens; VETERINARY vaccines; THEILERIA; THEILERIOSIS; VACCINATION
- Publication
Tropical Medicine & International Health, 1999, Vol 4, Issue 9, pA71
- ISSN
1360-2276
- Publication type
journal article
- DOI
10.1046/j.1365-3156.1999.00453.x