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- Title
Pollen-derived nonallergenic substances enhance Th2-induced IgE production in B cells.
- Authors
Oeder, S.; Alessandrini, F.; Wirz, O. F.; Braun, A.; Wimmer, M.; Frank, U.; Hauser, M.; Durner, J.; Ferreira, F.; Ernst, D.; Mempel, M.; Gilles, S.; Buters, J. T. M.; Behrendt, H.; Traidl‐Hoffmann, C.; Schmidt‐Weber, C.; Akdis, M.; Gutermuth, J.
- Abstract
Background B cells play a central role in IgE-mediated allergies. In damaged airway epithelium, they are exposed directly to aeroallergens. We aimed to assess whether direct exposure of B cells to pollen constituents affects allergic sensitization. Methods B cells from murine splenocytes and from blood samples of healthy donors were incubated for 8 days under Th2-like conditions with aqueous ragweed pollen extracts (Amb- APE) or its constituents. Secreted total IgM, IgG, and IgE was quantified by ELISA. Additionally, birch, grass, or pine-pollen extracts were tested. The number of viable cells was evaluated by ATP measurements. B-cell proliferation was measured by CFSE staining. IgE class switch was analyzed by quantitation of class switch transcripts. In an OVA/Alum i.p.-sensitization mouse model, Amb- APE was intranasally instilled for 11 consecutive days. Results Upon Th2 priming of murine B cells, ragweed pollen extract caused a dose-dependent increase in IgE production, while IgG and IgM were not affected. The low-molecular-weight fraction and phytoprostane E1 ( PPE1) increased IgE production, while Amb a 1 did not. PPE1 enhanced IgE also in human memory B cells. Under Th1 conditions, Amb- APE did not influence immunoglobulin secretion. The IgE elevation was not ragweed specific. It correlated with proliferation of viable B cells, but not with IgE class switch. In vivo, Amb- APE increased total IgE and showed adjuvant activity in allergic airway inflammation. Conclusions Aqueous pollen extracts, the protein-free fraction of Amb- APE, and the pollen-contained substance PPE1 specifically enhance IgE production in Th2-primed B cells. Thus, pollen-derived nonallergenic substances might be responsible for B-cell-dependent aggravation of IgE-mediated allergies.
- Subjects
IMMUNOGLOBULIN E; B cells; TH2 cells; POLLEN; IMMUNOLOGICAL adjuvants; RESPIRATORY allergy; ALLERGY treatment; PHYSIOLOGY
- Publication
Allergy, 2015, Vol 70, Issue 11, p1450
- ISSN
0105-4538
- Publication type
Article
- DOI
10.1111/all.12707