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- Title
Sex differences in fractalkine responses in spontaneously hypertensive rats (SHR).
- Authors
Pardieck, Jennifer Lynn; Sullivan, Jennifer C.; Pollock, Jennifer S.
- Abstract
Lower cardiovascular risk in females has been linked to a sex difference in NO bioavailability. Fractalkine (FKN) has been shown to stimulate NOS to produce NO. We designed experiments to test the hypothesis that females have greater FKN-stimulated, NOS-mediated vasorelaxation. The mesenteric arterial bed (MAB) was isolated for measurement of FKN by RT-PCR and for FKN ligand and receptor by Western blot. Third order mesenteric arteries from male and female SHR (12-14 weeks) were isolated, and a cumulative dose-response curve was performed to acetylcholine (Ach) in arteries pre-incubated with FKN (1 rag/mL) or vehicle in the absence or presence of the NOS inhibitor Nω-nitro-L-arginine methyl ester (LNAME; 100 µM). There was greater FKN expression in the MAB of females when measured by RT-PCR (RDU: males, 0.2±0.1; females, 2.2±0.5, p=0.02), but FKN ligand and receptor protein expression were similar. LNAME produced a similar decrease in sensitivity and maximum response to Ach in arteries from males and females. Pre-incubation with FKN alone did not alter Ach-induced relaxation; however, incubation with FKN and LNAME produced a greater decrease in maximum response in males compared to females. These data suggest that despite increased FKN mRNA in arteries from females, FKN potentiates Ach-stimulated NO production to a greater extent in arteries from males.
- Subjects
SEX differences (Biology); HYPERTENSION; NITRIC oxide; LABORATORY rats; WESTERN immunoblotting; ACETYLCHOLINE
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1418
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.6.a1418