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- Title
Is the ClC-2 chloride channel involved in the Cl<sup>-</sup> secretory mechanism of gastric parietal cells?
- Authors
Hori, Katsuhito; Takahashi, Yuji; Horikawa, Naoki; Furukawa, Tetsushi; Tsukada, Kazuhiro; Takeguchi, Noriaki; Sakai, Hideki
- Abstract
It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl- channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H+,K+-ATPase. These results indicate that ClC-2 may not be a Cl--transporting protein for gastric acid secretion in parietal cells.
- Subjects
CHLORIDES; CELLS; IMMUNOHISTOCHEMISTRY; GASTRIC acid
- Publication
FEBS Letters, 2004, Vol 575, Issue 1-3, p105
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.08.044