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- Title
Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates.
- Authors
Mutai, Ivy Jepkurui; Georges, Martin; Nyachieo, Atunga; Odoyo, Eric; Nyamache, Anthony Kebira; Ngalla, Jillani
- Abstract
Objectives: The use of bacteriophages (phages) as an alternative treatment for multidrug-resistant bacteria has recently gained popularity. Phage cocktails have been proposed for broad-spectrum therapeutic effects against such resistant bacteria. However, the effectiveness of non-locally formulated phage cocktails for therapy on Kenyan isolates has yet to be evaluated and is a subject of investigation. This study aimed to determine the invitro effectiveness of a non-locally made commercial pyophage cocktail on clinical Pseudomonas aeruginosa isolates from Kenya. Methodology: Forty-nine P. aeruginosa isolates from Kenya were subjected to a pyophage cocktail for efficacy studies using direct spot test (DST) and efficiency of plating (EOP). Results: The success rate of the cocktail was observed on 16.3% (8/49) isolates only and ineffective on 83.7% (41/49) isolates. Of the eight isolates that showed cross-reactivity from DST, six had complete lysis with a faintly hazy background. Five of these six isolates resulted in successful and high phage progeny production in plaquing efficiency (EOP = 0.5). Conclusion: Non-locally made commercial pyophage cocktail was ineffective against the 83.7% endemic clinical strains of the Kenyan P. aeruginosa isolates, demonstrating the importance of locally derived phage cocktails against endemic and multidrug-resistant isolates.
- Subjects
KENYA; BACTERIOPHAGES; PSEUDOMONAS aeruginosa; CROSS reactions (Immunology)
- Publication
Journal of Microbiology & Infectious Diseases, 2023, Vol 13, Issue 1, p25
- ISSN
2146-3158
- Publication type
Article
- DOI
10.5799/jmid.1265378