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- Title
Simultaneous analysis of 210 prohibited substances in human urine by ultrafast liquid chromatography/tandem mass spectrometry in doping control.
- Authors
Jeong, Eun Sook; Kim, So‐Hee; Cha, Eun‐Ju; Lee, Kang Mi; Kim, Ho Jun; Lee, Sang‐Won; Kwon, Oh‐Seung; Lee, Jaeick
- Abstract
RATIONALE: Doping analysis is a two-step process consisting of a screening step for prohibited substances and a confirmation step to verify the presence of specific substances found during the screening. The entire process must be performed within a limited time period, but traditional screening procedures commonly employ separate analytical methods for each class of prohibited substances being screened and thus require a great deal of human resources and instrumentation. A single simple and rapid multiresidue analytical method that could accommodate multiple classes of prohibited substances would be extraordinarily useful in doping analyses. METHODS: Urine samples were extracted via two consecutive liquid-liquid extractions at different pH values following enzymatic hydrolysis. Analyses were performed by ultrafast liquid chromatography/triple-quadrupole mass spectrometry with polarity switching and time-dependent selected reaction monitoring. RESULTS:We developed a rapid multiresidue screening and confirmation method for efficient high-throughput doping analyses. The present method was validated with regard to the limits of detection (0.01-100.0 ng/mL for screening analyses and 0.2-500.0 ng/mL for confirmation assays), matrix effects (48.9-118.9%), recovery (20.6-119.7%) and intra- (0.6-17.6%) and inter-day (4.0-20.0%) precision. CONCLUSIONS: A multiresidue analytical method was developed and validated for screening and confirming the presence of performance-enhancing drugs. A total of 210 substances from diverse classes of prohibited substances were successfully identified with an analytical run time of 10 min.
- Subjects
URINE; LIQUID chromatography; TANDEM mass spectrometry; MASS spectrometry; HYDROLYSIS
- Publication
Rapid Communications in Mass Spectrometry: RCM, 2015, Vol 29, Issue 4, p367
- ISSN
0951-4198
- Publication type
Article
- DOI
10.1002/rcm.7113