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- Title
Healthy Elderly People Lack Neutrophil-Mediated Functional Activity to Type V Group B Streptococcus.
- Authors
Amaya, Rene A.; Baker, Carol J.; Keitel, Wendy A.; Edwards, Morven S.
- Abstract
To determine the function of capsular polysaccharide (CPS)-specific immunoglobulin-G (IgG) and neutrophils from older adults in increasing ingestion and killing of type V group B Streptococcus (GBS). Cross-sectional study. Outpatient clinic at Baylor College of Medicine. The subjects were 40 healthy, community-dwelling adults aged 65 and older from Houston, Texas. The serum level of type V GBS CPS-specific IgG was measured using an enzyme-linked immunosorbent assay. Functional activity was evaluated using an opsonophagocytosis assay. Sera from four subjects promoted efficient neutrophil-mediated phagocytosis and killing of type V GBS (mean log10 reduction±standard deviation in colony-forming units (cfu)=1.51±0.39). Each had serum CPS-specific IgG concentrations exceeding 1 μg/mL. Sera from 36 subjects did not promote neutrophil-mediated functional activity (mean log10 reduction in cfu=−0.09±0.06; P=.025). Only one of these 36 had a CPS-specific IgG concentration exceeding 1 μg/mL. When pooled sera from young adults given type V GBS conjugate vaccine was added at CPS-specific IgG concentrations of 4 μg/mL or 0.4 μg/mL, sera from all subjects promoted neutrophil-mediated killing of type V GBS. No impairment was evident in the neutrophil function of elderly subjects when it was compared with that of young adults. CPS-specific IgG and neutrophils from healthy older adults function to ingest and kill type V GBS, but these antibodies are not present in sufficient amounts in most individuals. Further studies should determine whether a type V GBS vaccine induces functionally active antibodies in older people.
- Subjects
IMMUNOGLOBULIN G; NEUTROPHILS; OLDER people; INGESTION; STREPTOCOCCUS; VACCINES
- Publication
Journal of the American Geriatrics Society, 2004, Vol 52, Issue 1, p46
- ISSN
0002-8614
- Publication type
Article
- DOI
10.1111/j.1532-5415.2004.52009.x