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- Title
Evaluation of Six Methods for Extraction of Total RNA from Loquat.
- Authors
Zhang LING; Zhang ZHIKE; Lin SHUNQUAN; Zheng TINGTING; Yang XIANGHUI
- Abstract
Trizol extraction for polysaccharide-rich plant tissue was unsuitable for isolating total RNA from loquat fruit. CTAB-LiCl extraction was improved with pretreatment using washing buffer with S0% ethanol and 70% acetone. The RNA isolated by this protocol from different loquat fruit tissue at various development periods, was high in purity (A260/A280 ratio ranged from 1.81 to 1.99 and A260/A230 ratio was over 2.0), and high in yield.
- Subjects
LOQUAT; POLYSACCHARIDES; EXTRACTION (Chemistry); PLANT cells &; tissues; ETHANOL; PLANT RNA; ACETONE
- Publication
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2013, Vol 41, Issue 1, p313
- ISSN
0255-965X
- Publication type
Article
- DOI
10.15835/nbha4118233