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- Title
Immunization with autologous T cells enhances in vivo anti-tumor immune responses accompanied by up-regulation of GADD45β.
- Authors
Li Wang; Fang Du; Qi Cao; Huiming Sheng; Baihua Shen; Yan Zhang; Yingna Diao; Jingwu Zhang; Ningli Li
- Abstract
Immunization with inactivated autoreactive T cells may induce idiotype anti-idiotypic reactions to deplete autoreactive T cells, which are involved in autoimmune diseases. However, it is unknown whether attenuated activated healthy autologous T-cell immunization could increase anti-tumor immune responses. To this end, C57Bl/6 mice were immunized with attenuated activated autologous T cells. The splenocytes from immunized mice showed a higher proliferative ability than that from naive mice. The special phenotype analysis showed that there were more CD8+ T cells and CD62L+ T cells in immunized mice after 24 h of culture with 10% fetal calf serum complete medium in vitro (P<0.01). These results demonstrated that this immunization may activate T cells in vivo. Furthermore, the splenocytes from immunized mice revealed resistance to activation-induced cell death (AICD) in vitro. To further study the relative genes that are responsible for the higher proliferation and resistance to AICD, the expression of Fas/Fas ligand (FasL) and GADD45b was measured by real-time PCR. The results indicated that GADD45β transcription was higher in the splenocytes from immunized mice than that in the naive mice. In addition, the Fas expression showed a parallel higher, but FasL did not change obviously. To investigate the biologic functions induced by immunization in vivo, a tumor model was established by EL-4 tumor cell inoculation in C57/Bl mice. Mice receiving autologous T-cell immunization had significantly inhibited tumor growth in vivo (P<0.01). This study implicated that immunization with attenuated activated autologous T cells enhances anti-tumor immune responses that participate in tumor growth inhibition.Cell Research (2006) 16: 702–712. doi:10.1038/sj.cr.7310083; published online 4 July 2006
- Subjects
IMMUNIZATION; T cells; LABORATORY mice; TUMOR immunology; IMMUNE response; AUTOIMMUNE diseases; PHENOTYPES
- Publication
Cell Research, 2006, Vol 16, Issue 8, p702
- ISSN
1001-0602
- Publication type
Article
- DOI
10.1038/sj.cr.7310083