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- Title
Effects of Purified Vitexin and Iso-Vitexin from Mung Bean Seed Coat on Antihyperglycemic Activity and Gut Microbiota in Overweight Individuals' Modulation.
- Authors
Yutharaksanukul, Pornlada; Tangpromphan, Preuk; Tunsagool, Paiboon; Sae-tan, Sudathip; Nitisinprasert, Sunee; Somnuk, Surasawadee; Nakphaichit, Massalin; Pusuntisumpun, Nut; Wanikorn, Bandhita
- Abstract
Exceeding a healthy weight significantly elevates the likelihood of developing type 2 diabetes (T2DM). A commercially available singular constituent, available as either purified vitexin or iso-vitexin, has been associated with a decreased risk of T2DM, but its synergistic effect has not been reported yet. Vitexin and iso-vitexin were extracted using an ethanol-based solvent from mung bean seed coat (MBCE) and subsequently purified using preparative liquid chromatography (Prep-LC). Eleven mixture ratios of vitexin and/or iso-vitexin were determined for their antioxidant and antihyperglycemic activities. The 1:1.5 ratio of vitexin to iso-vitexin from MBCE demonstrated the most synergistic effects for enzyme inhibition and glucose uptake in HepG2 cells within an insulin-resistant system, while these ratios exhibited a significantly lower antioxidant capacity than that of each individual component. In a gut model system, the ratio of 1:1.5 (vitexin and iso-vitexin) regulated the gut microbiota composition in overweight individuals by decreasing the growth of Enterobacteriaceae and Enterococcaceae, while increasing in Ruminococcaceae and Lachnospiraceae. The application of vitexin/iso-vitexin for 24 h fermentation enhanced a high variety of abundances of 21 genera resulting in five genera of Parabacteroides, Ruminococcus, Roseburia, Enterocloster, and Peptacetobacter, which belonged to the phylum Firmicutes, exhibiting high abundant changes of more than 5%. Only two genera of Proteus and Butyricicoccus belonging to Proteobacteria and Firmicutes decreased. The findings suggest that these phytochemicals interactions could have synergistic effects in regulating glycemia, through changes in antihyperglycemic activity and in the gut microbiota in overweight individuals. This optimal ratio can be utilized by industries to formulate more potent functional ingredients for functional foods and to create nutraceutical supplements aimed at reducing the risk of T2DM in overweight individuals.
- Subjects
HIGH performance liquid chromatography; RESEARCH funding; T-test (Statistics); GUT microbiome; HYPOGLYCEMIC agents; TREATMENT effectiveness; PHYTOCHEMICALS; FUNCTIONAL foods; DESCRIPTIVE statistics; PLANT extracts; SEEDS; FLAVONES; SOLVENTS; INSULIN resistance; TYPE 2 diabetes; ANTIOXIDANTS; ONE-way analysis of variance; DATA analysis software; OBESITY; LEGUMES; DISEASE risk factors
- Publication
Nutrients, 2024, Vol 16, Issue 17, p3017
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu16173017