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- Title
Assessment of transthyretin instability in patients with wild-type transthyretin amyloid cardiomyopathy.
- Authors
Iino, Takuya; Nagao, Manabu; Tanaka, Hidekazu; Yoshikawa, Sachiko; Asakura, Junko; Nishimori, Makoto; Shinohara, Masakazu; Harada, Amane; Watanabe, Shunsuke; Ishida, Tatsuro; Hirata, Ken-ichi; Toh, Ryuji
- Abstract
The pathophysiology of variant transthyretin (TTR) amyloidosis (ATTRv) is associated with destabilizing mutations in the TTR tetramer. However, why TTR with a wild-type genetic sequence misfolds and aggregates in wild-type transthyretin amyloidosis (ATTRwt) is unknown. Here, we evaluate kinetic TTR stability with a newly developed ELISA system in combination with urea-induced protein denaturation. Compared with that in control patients, endogenous TTR in patients with wild-type transthyretin amyloid cardiomyopathy (ATTRwt-CM) exhibited thermodynamic instability, indicating that circulating TTR instability may be associated with the pathogenesis of ATTRwt as well as ATTRv. Our findings provide new insight into the underlying mechanisms of ATTRwt.
- Subjects
TRANSTHYRETIN; AMYLOID; CARDIOMYOPATHIES; DENATURATION of proteins; AMYLOIDOSIS
- Publication
Scientific Reports, 2024, Vol 14, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-024-71446-8