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- Title
Effects of epigallocatechin gallate, caffeine, and their combination on fat accumulation in high-glucose diet-fed Caenorhabditis elegans.
- Authors
Cheng Guo; Wangyang Shen; Weiping Jin; Xiwu Jia; Zhili Ji; Li Jinling; Bin Li
- Abstract
Epigallocatechin gallate (EGCG) and caffeine are inevitable to be ingested together in the process of drinking green tea. This study used Caenorhabditis elegans as an organism model to examine whether the binding of EGCG and caffeine could influence the fat-reduction effect. The results revealed that EGCG significantly reduced the Nile Red fluorescence intensity and the triglyceride/protein ratio of the C. elegans obesity model by 14.7% and 16.5%, respectively, while the effect of caffeine was not significant. Moreover, the degree of reduction in fluorescence intensity and triglyceride/protein ratio by EGCG + caffeine was comparable to that of EGCG. In the exploration of underlying mechanism, we found that EGCG and EGCG + caffeine treatments had no influence on food intake and energy expenditure of C. elegans. Their fat-reduction effects were dependent on the regulation of lipogenesis, as shown by the decreased expression of the sbp-1, fat-7, and daf-16 genes.
- Subjects
EPIGALLOCATECHIN gallate; CAENORHABDITIS elegans; CAFFEINE; STAINS &; staining (Microscopy); CALORIC content of foods; GREEN tea; FAT
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 8, p898
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbad068