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- Title
Deletion of admB gene encoding a fungal ADAM affects cell wall construction in Aspergillus oryzae.
- Authors
Kobayashi, Takuji; Maeda, Hiroshi; Takeuchi, Michio; Yamagata, Youhei
- Abstract
Mammals possess a unique signaling system based on the proteolytic mechanism of a disintegrin and metalloproteinases (ADAMs) on the cell surface. We found two genes encoding ADAMs inAspergillus oryzaeand named themadmAandadmB. We producedadmAandadmBdeletion strains to elucidate their biological function and clarify whether fungal ADAMs play a similar role as in mammals. The∆admA∆admBand∆admBstrains were sensitive to cell wall-perturbing agents, congo red, and calcofluor white. Moreover, the two strains showed significantly increased weights of total alkali-soluble fractions from the mycelial cell wall compared to the control strain. Furthermore,∆admBshowed MpkA phosphorylation at lower concentration of congo red stimulation than the control strain. However, the MpkA phosphorylation level was not different between∆admBand the control strain without the stimulation. The results indicated thatA. oryzaeAdmB involved in the cell wall integrity without going through the MpkA pathway.
- Subjects
KOJI; FUNGAL cell walls; METALLOPROTEINASES
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 5, p1041
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2016.1270741