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- Title
Untitled.
- Authors
Pirard, Catherine; Focant, Jean-François; De Pauw, Edwin
- Abstract
The study and extension of a simple automated clean-up method for polychlorinated dibenzo- p-dioxins (PCDD) and polychlorinated dibenzofurans (PCDF) to a broad range of polychlorinated biphenyls (PCB) is described. The isolation of seven PCDD, ten PCDF, and three coplanar PCB (cPCB) is extended to eight mono- ortho substituted PCB and seven so-called "marker PCB" (Aroclor 1260) for fatty food samples. This enables quantification of 35 compounds – including all congeners with a WHO toxic equivalent factor (TEF) – in a single extraction and single purification step. The chromatographic behaviour of mono- ortho PCB and marker PCB on a variety of adsorbents, including basic alumina, has been studied. Partitioning of analytes through multi-column sequences is described and correlated with their structural and electronic properties, by use of molecular modelling calculations. The fractionation process available with the Power-Prep automated clean-up system enables rapid independent analysis of the different groups of compounds. Gas chromatography with high resolution mass spectrometry (GC–HRMS) is used for the PCDD/F and cPCB fraction and quadrupole ion-storage tandem in time mass spectrometry (GC–QISTMS) for analysis of the remaining PCB. A comparison study was performed on quality-control samples and real fatty food samples to evaluate the robustness of the new strategy compared with a reference method. On the basis of this simultaneous clean-up, a rapid simplified strategy for PCDD/F and selected PCB analysis determination is proposed for fatty food samples.
- Publication
Analytical & Bioanalytical Chemistry, 2002, Vol 372, Issue 2, p373
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-001-1181-y