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- Title
Tracking the Dynamic Folding and Unfolding of RNA G‐Quadruplexes in Live Cells.
- Authors
Chen, Xiu‐Cai; Chen, Shuo‐Bin; Dai, Jing; Yuan, Jia‐Hao; Ou, Tian‐Miao; Huang, Zhi‐Shu; Tan, Jia‐Heng
- Abstract
Abstract: Because of the absence of methods for tracking RNA G‐quadruplex dynamics, especially the folding and unfolding of this attractive structure in live cells, understanding of the biological roles of RNA G‐quadruplexes is so far limited. Herein, we report a new red‐emitting fluorescent probe, <bold>QUMA‐1</bold>, for the selective, continuous, and real‐time visualization of RNA G‐quadruplexes in live cells. The applications of <bold>QUMA‐1</bold> in several previously intractable applications, including live‐cell imaging of the dynamic folding, unfolding, and movement of RNA G‐quadruplexes and the visualization of the unwinding of RNA G‐quadruplexes by RNA helicase have been demonstrated. Notably, our real‐time results revealed the complexity of the dynamics of RNA G‐quadruplexes in live cells. We anticipate that the further application of <bold>QUMA‐1</bold> in combination with appropriate biological and imaging methods to explore the dynamics of RNA G‐quadruplexes will uncover more information about the biological roles of RNA G‐quadruplexes.
- Subjects
FLUORESCENT probes; QUADRUPLEX nucleic acids; REACTION mechanisms (Chemistry); ALDEHYDES; FLUORESCENCE spectroscopy; FORMAMIDE
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 17, p4792
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201801999