We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Development and Validation of SCAR Markers Co-Segregating with an Agropyron Elongatum Derived Leaf Rust Resistance Gene Lr24 in Wheat.
- Authors
Gupta, S. K.; Charpe, A.; Koul, S.; Haque, Q. M. R.; Prabhu, K. V.
- Abstract
An Agropyron elongatum-derived leaf rust resistance gene Lr24 located on chromosome 3DL of wheat was tagged with six random amplified polymorphic DNA (RAPD) markers which co-segregated with the gene. The markers were identified in homozygous resistant F2 plants taken from a population segregating for leaf rust resistance generated from a cross between two near-isogenic lines (NILs) differing only for Lr24. Phenotyping was done by inoculating the plants with pathotype 77-5 of Puccinia triticina. To enable gene-specific selection, three RAPD markers (S1302609, S1326615 and OPAB-1388) were successfully converted to polymorphic sequence characterized amplified region (SCAR) markers, amplifying only the critical DNA fragments co-segregating with Lr24. The SCAR markers were validated for specificity to the gene Lr24 in wheat NILs possessing Lr24 in 10 additional genetic backgrounds including the Thatcher NIL, but not to 43 Thatcher NILs possessing designated leaf rust resistance genes other than Lr24. This indicated the potential usefulness of these SCAR markers in marker assisted selection (MAS) and for pyramiding leaf rust resistance genes in wheat.
- Subjects
WHEATGRASSES; WHEAT rusts; DISEASE resistance of plants; PLANT chromosomes; RAPD technique; GENETIC markers; PLANT genetics
- Publication
Euphytica, 2006, Vol 150, Issue 1/2, p233
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-006-9113-8