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- Title
Production of ajmalicine and ajmaline in hairy root cultures of Rauvolfia micrantha Hook f., a rare and endemic medicinal plant.
- Authors
Sudha, C.G.; Reddy, B. Obul; Ravishankar, G.A.; Seeni, S.
- Abstract
Hairy roots of Rauvolfia micrantha were induced from hypocotyl explants of 2–3 weeks old aseptic seedlings using Agrobacterium rhizogenes ATCC 15834. Hairy roots grown in half-strength Murashige & Skoog (MS) medium with 0.2 mg indole 3-butyric acid l-1 and 0.1 mg α-naphthaleneacetic acid l-1 produced more ajmaline (0.01 mg g-1 dry wt) and ajmalicine (0.006 mg g-1 dry wt) than roots grown in auxin-free medium. Ajmaline (0.003 mg g-1 dry wt) and ajmalicine (0.0007 mg g-1 dry wt) were also produced in normal root cultures. This is the first report of production of ajmaline and ajmalicine in hairy root cultures of Rauvolfia micrantha.
- Subjects
RAUVOLFIA; MEDICINAL plants; ENDEMIC plants; PLANT roots; AGROBACTERIUM
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 8, p631
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1023012114628