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- Title
The first immunoglobulin-like domain of porcine nectin-1 is sufficient to confer resistance to pseudorabies virus infection in transgenic mice.
- Authors
Ono, E.; Tomioka, Y.; Watanabe, Y.; Amagai, K.; Taharaguchi, S.; Glenisson, J.; Cherel, P.
- Abstract
Nectin-1 is an alphaherpesvirus receptor that binds to virion glycoprotein D (gD). Porcine nectin-1 mediates entry of pseudorabies virus (PRV), herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), and bovine herpesvirus type 1 (BHV-1). The gD-binding domain of nectin-1 is the first or N-terminal immunoglobulin (Ig)-like domain of the entire ectodomain. Here, we generated three transgenic mouse lines expressing a fusion protein consisting of the first Ig-like domain of porcine nectin-1 and the Fc portion of porcine IgG1 to assess the antiviral potential of the first Ig-like domain of nectin-1 in vivo. All of the transgenic mouse lines showed significant resistance to PRV infection via intraperitoneal inoculation (survival rates of 67% to 100%). In the intranasal challenge, a lower but still significant protection was observed; 21% to 55% of the animals from the three transgenic mouse lines survived. The present results demonstrate that a soluble form of the first domain of porcine nectin-1 is able to exert a significant antiviral effect against pseudorabies virus infection.
- Subjects
AUJESZKY'S disease virus; TRANSGENIC mice; IMMUNOGLOBULINS; HERPES simplex virus; HERPESVIRUSES; GLYCOPROTEINS; INFECTION
- Publication
Archives of Virology, 2006, Vol 151, Issue 9, p1827
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-006-0747-6