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- Title
Multiple glutathione S-transferase genes: identification and expression in oriental fruit fly, Bactrocera dorsalis.
- Authors
Hu, Fei; Dou, Wei; Wang, Jing‐Jing; Jia, Fu‐Xian; Wang, Jin‐Jun
- Abstract
Background The oriental fruit fly, Bactrocera dorsalis (Hendel), is widely distributed in Asia-Pacific regions, where it is a serious pest of a wide range of tropical and subtropical fruit and vegetable crops. In this study, 17 cDNA encoding glutathione S-transferases ( GSTs) in B. dorsalis were sequenced and characterised. Results Phylogenetic analysis revealed that 16 GSTs belonged to five different cytosolic classes, including four in delta, eight in epsilon, two in omega, one in theta, and one in zeta. The remaining GST ( BdGSTu1) was unclassified. RT-qPCR assay showed that the relative expression levels of five GST genes were significantly higher in larval stages than in adulthood. Tissue-specific expression analysis found that BdGSTe3, BdGSTe9 and BdGSTd5 were expressed highly in the midgut, BdGSTe4, BdGSTe6, BdGSTd6 and BdGSTz2 were higher in the fat body, and six GSTs were higher in Malpighian tubules. RT-qPCR confirmed that the expressions of nine GST genes were increased by malathion exposure at various times and doses, while BdGSTe4, BdGSTe9 and BdGSTt1 were increased by β-cypermethrin exposure. Conclusion The increases in GST gene expression levels after malathion and β-cypermethrin exposure in B. dorsalis might increase the ability of this species to detoxify other insecticides and xenobiotics. © 2013 Society of Chemical Industry
- Subjects
ORIENTAL fruit fly; INSECTICIDE application; ANTISENSE DNA; INSECT phylogeny; CYPERMETHRIN; XENOBIOTICS; GENE expression
- Publication
Pest Management Science, 2014, Vol 70, Issue 2, p295
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.3558