We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Sequencing SARS-CoV-2 genomes from saliva.
- Authors
Alpert, Tara; Vogels, Chantal B F; Breban, Mallery I; Petrone, Mary E; Wyllie, Anne L; Grubaugh, Nathan D; Fauver, Joseph R
- Abstract
Genomic sequencing is crucial to understanding the epidemiology and evolution of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Often, genomic studies rely on remnant diagnostic material, typically nasopharyngeal (NP) swabs, as input into whole-genome SARS-CoV-2 next-generation sequencing pipelines. Saliva has proven to be a safe and stable specimen for the detection of SARS-CoV-2 RNA via traditional diagnostic assays; however, saliva is not commonly used for SARS-CoV-2 sequencing. Using the ARTIC Network amplicon-generation approach with sequencing on the Oxford Nanopore MinION, we demonstrate that sequencing SARS-CoV-2 from saliva produces genomes comparable to those from NP swabs, and that RNA extraction is necessary to generate complete genomes from saliva. In this study, we show that saliva is a useful specimen type for genomic studies of SARS-CoV-2.
- Subjects
SARS-CoV-2; SALIVA
- Publication
Virus Evolution, 2022, Vol 8, Issue 1, p1
- ISSN
2057-1577
- Publication type
Article
- DOI
10.1093/ve/veab098