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- Title
薄壳山核桃品种亲缘关系分析与 指纹图谱构建.
- Authors
何旭东; 郑纪伟; 田雪瑶; 教忠意; 窦全琴
- Abstract
[Objective] Since the morphological appearances among pecan(Carya illinoensis) varieties are extremely similar due to the narrow genetic basis of the crossing parents, making it difficult to precisely identify by phenotypic characters only, this study aims at elucidating the genetic relationships and constructing fingerprint among pecan varieties, and establishing a rapid, efficient, stable, and accurate genotyping system for pecan based on fluorescent SSR markers combining high throughput capillary electrophoresis technology. [Method] A total of 54 SSR primers were selected from pecan and related species for preliminary screening and ten of them were labeled by fluorophore for further analysis. 25 pecan varieties were genotyped by capillary electrophoresis, and the number of allele(A) and polymorphic information content(PIC) of each locus was scored and calculated by software. The fingerprint of pecan varieties was constructed using different combination of SSR markers and the genetic relationships among varieties were also investigated through cluster analysis. [Result] A total of 68 alleles were detected by ten pairs of SSR markers with an average of 6.8 alleles. The most(12 alleles) were screened at the Cc19 locus and the least(3 alleles) were obtained at the BFU-Jr locus. The polymorphic information content varied with locus from 0.291 0 to 0.843 5(mean 0.588 3). With the four optimal pairs of core primers, all of the 25 pecan varieties could be completely distinguished by the primer combinations of Cc19, PM-GA31, PM-CIN4, and PM-GA41. The cluster analysis demonstrated that the similarity coefficient of the 25 pecan varieties varied from 0.62 to 0.99. Two main clades were formed, in which some related varieties could be clustered together and some of them could not be completely corresponding with genetic background. [Conclusion] Compared with traditional dominant markers and polyacrylamide gel electrophoresis, the genotyping technology consisting of fluorescence-labeled SSR primers and capillary electrophoresis is quite practical with high throughput, fast speed, and stable and reliable result. The different varieties can be efficiently identified by the combination of core markers. In order to deeply reveal the real architecture of genetic relationship among pecan varieties, it is suggested that more crossing parents should be added and a certain number of more efficient markers covered the whole genome need to be selected during the analysis of genetic relationship.
- Publication
Forest Research, 2021, Vol 34, Issue 4, p95
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2021.04.011