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- Title
不同沙棘品种种子中α-亚麻酸含量 差异及相关基因分析.
- Authors
张 彤; 吕中睿; 魏继华; 张国昀; 罗红梅; 何彩云
- Abstract
[Objective] To study the differences of gene regulation in seeds of two sea buckthorn (Hippophae rhamnoides) cultivars with different α-linolenic acid content and to find the method of further increase the content of α-linolenic acid in H. rhamnoides seed. [Method] The difference in α-linolenic acid content and the expressed genes in the seeds of two H. rhamnoides cultivars were analyzed and determined by gas chromatography-mass spectrometry and transcriptomics. [Result] The proportion of α-linolenic acid in H. rhamnoides 'Mongolia' (XY) and H. rhamnoides ‘Sinensis’ (FN) seeds was different. The fatty acid with the highest content of XY was α-linolenic acid, followed by linoleic acid. The content of α-linolenic acid in FN was lower than that of linoleic acid. In semi-mature stage (T2), the expression levels of gene FAD2 and gene FAD3 in XY were 3.83 times and 13.63 times that in FN respectively. The expression of gene FAD7 in XY was 2.09 times and 1.72 times that in FN in immature stage (T1) and T2respectively. It was consistent with the trend of linoleic acid and α-linolenic acid content. On the contrary, the expressions of gene LOX3.1 and gene LOX3.2 in FN were 8.02 times and 7.12 times that of XY in T2 respectively. [Conclusion] The high accumulation of α-linolenic acid in H. rhamnoides seeds is due to the synergistic effect of multiple genes. High expression of gene FAD2, FAD3, FAD7 and low expression of gene LOX3.1, LOX3.2 contribute to the accumulation of α-linolenic acid.
- Subjects
MONGOLIA; HIPPOPHAE rhamnoides; GENETIC regulation; SEA buckthorn; GENE expression; FATTY acids; LINOLENIC acids; LINOLEIC acid
- Publication
Forest Research, 2020, Vol 33, Issue 4, p1
- ISSN
1001-1498
- Publication type
Article
- DOI
10.13275/j.cnki.lykxyj.2020.04.001