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- Title
Lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells improve erectile function in a rat model of bilateral cavernous nerve injury.
- Authors
Zhang, Zhenbin; Nie, Pan; Yang, Wende; Ma, Xiaolei; Chen, Zehong; Wei, Hongbo
- Abstract
Background: Erectile dysfunction (ED) often occurs due to cavernous nerve injury (CNI) after colorectal surgery. Cell-based therapies have great potential for the treatment of CNI-related ED; however, it needs to be optimised. In this study, we explored the therapeutic effects of lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells (L-ADSCs) on CNI-induced ED in rats. Results: The results of this in vitro study revealed that low-dose lipopolysaccharide could increase the viability of ADSCs, inhibit caspase 3 activation induced by hydrogen peroxide and promote cell migration. Compared with the ADSC supernatant, the L-ADSC supernatant could better reduce fibrosis in the corpus cavernosum smooth muscle cells induced by transforming growth factor-beta 1 protein. In the in vivo study, it was compared to ADSCs therapy, where the L-ADSCs therapy indicated that could better improve erectile function by increasing smooth muscle content and alleviating penile fibrosis in rats 2 weeks after CNI. The outcome may be related to the increase in the hepatocyte growth factor content in the corpus cavernosum and myelin basic protein in the major pelvic ganglion. Conclusions: L-ADSC treatment may be a promising approach for restoring erectile function after CNI.
- Subjects
STEM cells; NERVOUS system injuries; MYELIN basic protein; HEPATOCYTE growth factor; STEM cell transplantation; ANIMAL disease models; ELLAGIC acid
- Publication
Basic & Clinical Andrology, 2022, Vol 32, Issue 1, p1
- ISSN
2051-4190
- Publication type
Article
- DOI
10.1186/s12610-022-00156-w