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- Title
Serum microRNAs levels in primary focal segmental glomerulosclerosis.
- Authors
Cai, Xiaoyi; Xia, Zhengkun; Zhang, Chunni; Luo, Yang; Gao, Yuanfu; Fan, Zhongmin; Liu, Mengyuan; Zhang, Ying
- Abstract
Background: MicroRNAs (miRNAs, miRs) are involved in most physiological, developmental, and pathological processes. miR-192 and miR-205 are expressed preferentially in the renal cortex and closely relevant to the renal cell biology. In the present study, we aim to measure the serum levels of miR-192 and miR-205 and their correlation with clinicopathological data in patients with primary focal segmental glomerulosclerosis (FSGS) and minimal change disease (MCD). Methods: Fifty-six patients (35 male, 21 female) with idiopathic nephrotic syndrome (FSGS 30, MCD 26) and 20 healthy controls were enrolled in the study. We quantified the serum levels of miR-192 and miR-205 in patients with FSGS and MCD by RT-qPCR. Results: Patients with FSGS had higher serum levels of miR-192 and miR-205 than those with MCD (324.49 ± 42.74 fmol/l versus 90.19 ± 27.14 fmol/l, p < 0.01, 2.25 ± 0.69 fmol/l versus 0.60 ± 0.51 fmol/l, p < 0.01, respectively). The level of miR-192 was positively correlated with the proteinuria in patients with FSGS and MCD ( r = 0.62, p < 0.001, r = 0.84, p < 0.001, respectively). Similarly, the level of miR-205 was positively correlated with the proteinuria in patients with FSGS ( r = 0.54, p = 0.002). In addition, the serum level of miR-192 was significantly correlated with the degree of interstitial fibrosis in patients with FSGS ( r = 0.342, p < 0.05). Conclusions: miR-192 and miR-205 have the potential as markers to differentiate FSGS from MCD.
- Subjects
NEPHROTIC syndrome diagnosis; ANALYSIS of variance; BIOMARKERS; CHI-squared test; POLYMERASE chain reaction; RESEARCH funding; RNA; STATISTICAL hypothesis testing; T-test (Statistics); CONTROL groups; REVERSE transcriptase polymerase chain reaction
- Publication
Pediatric Nephrology, 2013, Vol 28, Issue 9, p1797
- ISSN
0931-041X
- Publication type
Article
- DOI
10.1007/s00467-013-2434-7