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- Title
Microbial diversity of the Soldhar hot spring, India, assessed by analyzing 16S rRNA and protein-coding genes.
- Authors
Sharma, Avinash; Jani, Kunal; Shouche, Yogesh; Pandey, Anita
- Abstract
Bacterial diversity of the Soldhar hot spring, located in the Chamoli district of Uttarakhand, India, was investigated using a clone library, denaturing gradient gel electrophoresis (DGGE) and functional genes. Physicochemical analysis of sediment samples indicated an oligotrophic environment with very low sulfur content. Based on the 16S rRNA gene studies Proteobacteria was the most predominant group in all the three approaches. Other dominant phyla were Deinococcus- Thermus and Aquificae. Pyrobaculum was the only archaeal genus detected by DGGE. In the functional gene analysis, the nifH library showed a single operational taxonomic unit (OTU) related to the genus Paenibacillus whereas the aoxB library showed three OTUs related to Acidovorax, Aminobacter and Agrobacterium. Our results demonstrate for the first time both the bacterial and archaeal diversity in the Soldhar hot spring by culture-independent techniques, thereby providing important information that will increase our understanding of the microbial ecology of the Soldhar hot spring.
- Publication
Annals of Microbiology, 2015, Vol 65, Issue 3, p1323
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/s13213-014-0970-4