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- Title
Impaired autophagy flux by lncRNA NEAT1 is critical for inflammation factors production in human periodontal ligament stem cells with nicotine treatment.
- Authors
Taotao Zhang; Kuan Yang; Yujiang Chen; Yuran Jiang; Zhifei Zhou; Jiajia Liu; Yang Du; Lulu Wang; Xinxin Han; Xingan Wu; Xiaojing Wang
- Abstract
Background and Objectives: Periodontitis is the top reason for tooth loss, and smoking significantly increases severe periodontitis risk. Defective autophagy has been reported to play a vital role in periodontitis. This study aimed to elucidate the relationship between autophagy and inflammation factors production in nicotine-treated periodontal ligament stem cells (PDLSCs) and the underlying mechanism. Methods: In this study, transmission electron microscopy, immunofluorescence, and the mCherry-GFP- LC3 plasmid were used to study autophagy flux. The gene levels of inflammation factors and long noncoding RNA nuclear paraspeckle assembly transcript 1 (lncRNA NEAT1) were detected by quantitative real-time PCR (qRT-PCR). Western blot was performed to assess the protein levels of autophagic markers and α7 nicotinic acetylcholine receptor (α7nAChR). Results: We found that nicotine impaired autophagosome-lysosome fusion and lysosome functions to block autophagy flux, contributing to inflammatory factors production in nicotine-treated PDLSCs. Moreover, nicotine upregulated NEAT1 by activating α7nAChR. NEAT1 decreased autophagy flux by downregulating syntaxin 17 (STX17). Conclusion: Our data indicate that NEAT1-decreased autophagy flux is pivotal for inflammation factors production in nicotine-treated PDLSCs.
- Subjects
RNA metabolism; THERAPEUTIC use of nicotine; REVERSE transcriptase polymerase chain reaction; LYSOSOMES; SEQUENCE analysis; PERIODONTITIS; AUTOPHAGY; INFLAMMATION; CHOLINERGIC receptors; WESTERN immunoblotting; ONE-way analysis of variance; ELECTRON microscopy; GENE expression; T-test (Statistics); STEM cells; FLUORESCENT antibody technique; GENE expression profiling; DESCRIPTIVE statistics; RESEARCH funding; MEMBRANE proteins; DATA analysis software; PERIODONTAL ligament
- Publication
Journal of Periodontal Research, 2023, Vol 58, Issue 1, p70
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/jre.13069