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- Title
The CD133+ tumor stem-like cell-associated antigen may elicit highly intense immune responses against human malignant glioma.
- Authors
Hua, Wei; Yao, Yu; Chu, Yiwei; Zhong, Ping; Sheng, Xiaofang; Xiao, Baoguo; Wu, Jingsong; Yang, Bojie; Mao, Ying; Zhou, Liangfu
- Abstract
To explore the immunogenicity of glioma stem-like cell-associated antigens (SAAs) from sorted or unsorted glioma tumor stem-like cells (TSCs) as well as irradiated TSCs. Two primary human malignant glioma lines (SHG62, SHG66) and U87 cell line were primarily cultured in the serum-free medium (SFM) supplemented with EGF/bFGF. TSCs were identified by their self-renewal, multi-lineage differentiation and tumorigenic activity. To prepare SAAs in vitro, CD133+ TSCs were sorted either by magnetic cell sorting or with irradiation (6 Gy).The cytotoxicity induced by autogenous myeloid dendritic cell (DC)-mediated SAA-specific cytotoxic T lymphocytes (CTLs) was assessed by the Just Another Method test. SHG62, SHG66, and U87 cells contained TSCs. CD133+ SAAs-specific CTLs were significantly more cytotoxic than effector cells loaded with unsorted SAA ( P < 0.05). Effector cells loaded with irradiated SAAs were more cyotoxic than those with regular SAAs ( P < 0.01). SAAs from CD133+ TSCs and irradiated TSCs provide highly immunogenic antigens. TSCs might be a novel source of antigens for DC vaccination against malignant gliomas.
- Subjects
ANTIGENS; IMMUNE response; GLIOMAS; CANCER cells; STEM cells; CELL lines; EPIDERMAL growth factor
- Publication
Journal of Neuro-Oncology, 2011, Vol 105, Issue 2, p149
- ISSN
0167-594X
- Publication type
Article
- DOI
10.1007/s11060-011-0572-y