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- Title
Optimization of media and submerged fermentation conditions using central composite design for increased endoglucanase production by Cladosporium sp. NCIM 901.
- Authors
Mohan, Poludasu Rama; Kumar, Poondla Vijaya; Reddy, Obulam Vijaya Sarathi
- Abstract
Objective: Present work was focused on utilization of lignocellulosic substrates like bagasse and citrus peel powder for CMCase (endoglucanase) production through submerged fermentation by Cladosporium sp. NCIM 901. Methods: Fungal strain Cladosporium sp. was procured from NCIM, Pune. Peracetic acid pretreated bagasse was used in order to reduce the crystallinity of bagasse. Mary Mandels medium was used for enzyme production. Central composite design was evaluated to study the effect of five variables on CMCase production and to optimize the medium composition. Results: Experimental results showed the optimal medium for higher enzyme production was composed of 10% (w/v) bagasse substrate, 4.5% (w/v) citrus peel powder, 0.17% (w/v) MgSO4, 0.17% (w/v) urea with the initial pH of medium at 5.5. Maximum enzyme yield (15.02 IU/ml) was obtained on by the interaction of optimal level of process involved parameters. There is a good correlation between actual and predicted results. The coefficient of determination (R2) is 0.9414. Conclusion: Use of cheap and environment friendly available agricultural and fruit waste substrates for enzyme production eventually reduces the production cost of enzymes. Peracetic acid pretreated bagasse was used with citrus peel powder as fermentation medium constituent to induce the enzyme production. Observed actual enzyme activity was well agreed with the predicted enzymatic activity, shown the model was applicable for enzyme production through submerged fermentation.
- Subjects
GLUCANASES; CLADOSPORIUM; LIGNOCELLULOSE; ENZYMES; AGRICULTURAL waste research; FERMENTATION
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2013, Vol 38, Issue 4, p385
- ISSN
0250-4685
- Publication type
Article
- DOI
10.5505/tjod.2013.39200