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- Title
Development of novel aptamers for low-density lipoprotein particle quantification.
- Authors
Klapak, Daniel; Broadfoot, Sarah; Penner, Gregory; Singh, Anup; Inapuri, Eshwar
- Abstract
Cardiovascular disease (CVD) remains the leading cause of death worldwide. Low-density lipoprotein cholesterol (LDL-C) is commonly used for CVD risk assessment; however, recent research has shown LDL particle (LDL-P) number to be a more sensitive indicator of CVD risk than both LDL-C and non-high-density lipoprotein cholesterol (HDL-C). Described herein are five single stranded DNA aptamers with dissociation constants in the low picomolar range specific to LDL-P and its subfractions. Furthermore, a set of antisense sequences have been developed and characterized that are capable of binding to the best aptamers and undergoing displacement by LDL-P for use in a simple, affordable diagnostic assay.
- Subjects
CARDIOVASCULAR diseases risk factors; LOW density lipoproteins; APTAMERS; CAUSES of death; SINGLE-strand DNA breaks
- Publication
PLoS ONE, 2018, Vol 13, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0205460