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- Title
Sucrose Ingestion Induces Rapid AMPA Receptor Trafficking.
- Authors
Tukey, David S.; Ferreira, Jainne M.; Antoine, Shannon O.; D'amour, James A.; Ninan, Ipe; Cabeza de Vaca, Soledad; Incontro, Salvatore; Wincott, Charlotte; Horwitz, Julian K.; Hartner, Diana T.; Guarini, Carlo B.; Khatri, Latika; Goffer, Yossef; Duo Xu; Titcombe, Roseann F.; Khatri, Megna; Marzan, Dave S.; Mahajan, Shahana S.; Jing Wang; Froemke, Robert C.
- Abstract
The mechanisms by which natural rewards such as sugar affect synaptic transmission and behavior are largely unexplored. Here, we investigate regulation of nucleus accumbens synapses by sucrose intake. Previous studies have shown that AMPA receptor (AMPAR) trafficking is a major mechanism for regulating synaptic strength, and that in vitro, trafficking of AMPARs containing the G1uA1 subunit takes place by a two-step mechanism involving extrasynaptic and then synaptic receptor transport. We report that in rat, repeated daily ingestion of a 25% sucrose solution transiently elevated spontaneous locomotion and potentiated accumbens core synapses through incorporation of Ca2+-permeable AMPA receptors (CPARs), which are GluA 1-containing, GluA2-lacking AMPARs. Electrophysiological, biochemical, and quantitative electron microscopy studies revealed that sucrose training (7 d) induced a stable (>24 h) intraspinous GluAl population, and that in these rats a single sucrose stimulus rapidly (5 mm) but transiently (<24h) elevated G1uA1 at extrasynaptic sites. CPARs and dopamine Dl receptors were required in vivo for elevated locomotion after sucrose ingestion. Significantly, a 7 d protocol of daily ingestion of a 3% solution of saccharin, a noncaloric sweetener, induced synaptic G1uA1 similarly to 25% sucrose ingestion. These findings identify multistep G1uA1 trafficking, previously described in vitro, as a mechanism for acute regulation of synaptic transmission in vivo by a natural orosensory reward. Trafficking is stimulated by a chemosensory pathway that is not dependent on the caloric value of sucrose.
- Subjects
NEURAL transmission; SYNAPSES; AMPA receptors; ELECTRON microscopy; SACCHARIN; CHEMORECEPTORS
- Publication
Journal of Neuroscience, 2013, Vol 33, Issue 14, p6123
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.4806-12.2013