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- Title
Behavioral and Synaptic Plasticity Are Impaired upon Lack of the Synaptic Protein SAP47.
- Authors
Saumweber, Timo; Weyhersmüller, Annika; Hallermann, Stefan; Diegelmann, Sören; Michels, Birgit; Bucher, Daniel; Funk, Natalja; Reisch, Dietmar; Krohne, Georg; Wegener, Stephanie; Buchner, Erich; Gerber, Bertram
- Abstract
The synapse-associated protein of 47kDa(SAP47) is a member of a phylogenetically conserved gene family of hitherto unknown function. In Drosophila, SAP47 is encoded by a single gene (Sap47) and is expressed throughout all synaptic regions of the wild-type larval brain; specifically, electron microscopy reveals anti-SAP47 immunogold labeling within 30 nm of presynaptic vesicles. To analyze SAP47 function, we used the viable and fertile deletion mutant Sap47156, which suffers from a 1.7 kb deletion in the regulatory region and the first exon. SAP47 cannot be detected by either immunoblotting or immunohistochemistry in Sap47156 mutants. These mutants exhibit normal sensory detection of odorants and tastants as well as normal motor performance and basic neurotransmission at the neuromuscular junction. However, short-term plasticity at this synapse is distorted. Interestingly, Sap47156 mutant larvae also show a 50% reduction in odorant-tastant associative learning ability; a similar associative impairment is observed in a second deletion allele (Sap47201) and upon reduction of SAP47 levels using RNA interference. In turn, transgenically restoring SAP47 in Sap47156 mutant larvae rescues the defect in associative function. This report thus is the first to suggest a function for SAP47. It specifically argues that SAP47 is required for proper behavioral and synaptic plasticity in flies-and prompts the question whether its homologs are required for proper behavioral and synaptic plasticity in other species as well.
- Subjects
SYNAPSES; PROTEINS; GENES; SYNAPTIC vesicles; OLFACTORY receptors; MATERIAL plasticity; SPECIES
- Publication
Journal of Neuroscience, 2011, Vol 31, Issue 9, p3508
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.2646-10.2011