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- Title
An engineered food-grade Lactococcus lactis strain for production and delivery of heat-labile enterotoxin B subunit to mucosal sites.
- Authors
Nan Sun; Rongguang Zhang; Guangcai Duan; Xiaoyan Peng; Chen Wang; Qingtang Fan; Shuaiyin Chen; Yuanlin Xi
- Abstract
Background: Recent researches have been focusing on mucosal immune adjuvants, which play the key roles in mucosal immunization and have become the limitation for non-injected vaccine development. Escherichia coli heatlabile enterotoxin B subunit (LTB) was regarded as a promising mucosal adjuvant for its nontoxicity and potent activity. LTB preparation issues have always been recurring, in part owing to that the recombinant LTB expressed by E. coli does not act as its native form. Results: We constructed an engineered Lactococcus lactis strain using a food-grade expression system. The LTB secreted by the engineered strain was detected in the culture supernatant, constituting 10.3% of the supernatant proteins, and recognized by mouse anti-LTB antibodies. The engineered strain, co-administered orally to SPF BALB/c mice with a H. pylori vaccine candidate expressing Lpp20 antigen, could significantly enhance the Lpp20-induced mucosal SIgA antibody responses against H. pylori. Conclusions: This is the first report that LTB was efficiently produced and delivered via using a food-grade lactococcal expression system, which offers a novel production and utilization mode of this crucial mucosal adjuvant. The engineered L. lactis strain secreting LTB has considerable potential for oral vaccine formulation owing to its outstanding safety, adjuvant activity and high-level production.
- Subjects
LACTOCOCCUS lactis; ENTEROTOXINS; IMMUNIZATION; ESCHERICHIA coli; ORAL vaccines; IMMUNOGLOBULINS
- Publication
BMC Biotechnology, 2017, Vol 17, p1
- ISSN
1472-6750
- Publication type
Article
- DOI
10.1186/s12896-017-0345-6