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- Title
Differential mRNA expression levels and gene sequences of carboxylesterase in both deltamethrin resistant and susceptible strains of the cotton aphid, Aphis gossypii.
- Authors
Chuan-Wang Cao; Jing Zhang; Xi-Wu Gao; Pei Liang; Hui-Lin Guo
- Abstract
Extensive use of insecticides on cotton has prompted resistance development in the cotton aphid, Aphis gossypii (Glover) in China. A deltamethrin-selected population of cotton aphids from Xinjiang Uygur Autonomous Region, China with 228.59-fold higher resistance to deltamethrin was used to examine how carboxylesterase conferred resistance to this pyrethroid insecticide. The carboxylesterase activity in the deltamethrin-resistant strain was 3.67-, 2.02- and 1.16-fold of the susceptible strain when using α-naphthyl acetate (α-NA), β-naphthyl acetate (β-NA) and α-naphthyl butyrate (α-NB) as substrates, respectively. Carboxylesterase cDNA was cloned and sequenced from both deltamethrin-resistant and susceptible strains. The cDNA contained 1581 bp open reading frames (ORFs) coding a 526 amino acid protein. Only one amino acid substitution (Val87-Ala) was observed between deltamethrin-resistant and susceptible strains but it is not genetically linked to resistance by the catalytic triad and signature motif analysis. The real-time polymerase chain reaction analysis indicated that the resistant strain had a 6.61-fold higher level of carboxylesterase mRNA than the susceptible strain. The results revealed that up-regulation of the carboxylesterase gene, not modified gene structure, may be responsible for the development of resistance in cotton aphids to deltamethrin.
- Subjects
XINJIANG Uygur Zizhiqu (China); CHINA; COTTON aphid; GENE expression; MESSENGER RNA; INSECTICIDE resistance; PYRETHROIDS; AMINO acids
- Publication
Insect Science, 2008, Vol 15, Issue 3, p209
- ISSN
1672-9609
- Publication type
Article
- DOI
10.1111/j.1744-7917.2008.00202.x