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- Title
Synthesis and Characterisation of Self-Assembled and Self-Adjuvanting Asymmetric Multi-Epitope Lipopeptides of Ovalbumin.
- Authors
Eskandari, Sharareh; Stephenson, Rachel J.; Fuaad, Abdullah Ahmad; Apte, Simon H.; Doolan, Denise L.; Toth, Istvan
- Abstract
Designing a lipopeptide (LP) vaccine with a specific asymmetric arrangement of epitopes may result in an improved display of antigens, increasing host-cell recognition and immunogenicity. This study aimed to synthesise and characterise the physicochemical properties of a library of asymmetric LP-based vaccine candidates that contained multiple CD4+ and CD8+ T-cell epitopes from the model protein antigen, ovalbumin. These fully synthetic vaccine candidates were prepared by microwave-assisted solid phase peptide synthesis. The C12 or C16 lipoamino acids were coupled to the N or C terminus of the OVA CD4 peptide epitope. The OVA CD4 LPs and OVA CD8 peptide constructs were then conjugated using azide-alkyne Huisgen cycloaddition to give multivalent synthetic vaccines. Physiochemical characterisation of these vaccines showed a tendency to self-assemble in aqueous media. Changes in lipid length and position induced self-assembly with significant changes to their morphology and secondary structure as shown by transmission electron microscopy and circular dichroism.
- Subjects
LIPOPEPTIDE antibiotics; VACCINES; EPITOPES; T cells; OVALBUMINS
- Publication
Chemistry - A European Journal, 2015, Vol 21, Issue 3, p1251
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201404997