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- Title
Ultra‐Fast Photonic Digital Polymerase Chain Reaction based on N‐Heterocyclic Carbene Self‐Assembled Monolayer.
- Authors
Kim, Kyung Ho; Seo, Sung Eun; Kim, Jinyeong; Park, Seon Joo; An, Jai Eun; Shin, Chan Jae; Ryu, Choong‐Min; Lee, Sung Woon; Nam, Ho Chul; Yoon, Tae Ho; Shin, Jong Cheol; Kim, Yu Kyung; Oh, Hanseul; Hong, Jung Joo; Kim, Brian N.; Lee, Kyoung G.; Song, Hyun Seok; Lee, Sang Hun; Kwon, Oh Seok
- Abstract
A molecular diagnosis of the respiratory syncytial virus (RSV) without bulky and expensive instrumentation is of great importance for the early detection and prevention in a fast‐spreading pandemic. However, the current representative diagnostic methods have the limitation of being time‐consuming, cost, the processing time for polymerase chain reaction (PCR), and inaccurate for lateral flow assay (LFA), representatively. Herein, an integrated photonic digital PCR (dPCR) is developed with high‐velocity photonic scanner for in situ fluorescence detection by introducing the N‐heterocyclic carbene self‐assembled monolayer‐based Au film to prevent the quenching effect. The on‐site rapid molecular diagnostic platform shows the driving of 40 cycles in under 8 min and fluorescence scanning in under 7 min, resulting in a total analysis time within 15 min. In particular, the technology clearly demonstrates the classification of SARS‐CoV‐2 patients and healthy controls (99% in sensitivity, 98.6% in specificity, and 96.4% in accuracy with RdRp gene), comparing with standard RT‐qPCR. This platform can be utilized for prompt point‐of‐care molecular diagnostics in early diagnosis and large‐scale prevention of next pandemic spreading for upcoming infectious diseases and for the distinction diagnosis with other RSV.
- Subjects
POLYMERASE chain reaction; RESPIRATORY syncytial virus; MOLECULAR diagnosis; MONOMOLECULAR films; EARLY diagnosis
- Publication
Advanced Functional Materials, 2023, Vol 33, Issue 37, p1
- ISSN
1616-301X
- Publication type
Article
- DOI
10.1002/adfm.202303728