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- Title
Anther culture of Sorghum bicolor (L.) Moench.
- Authors
Rose, Judith; Dunwell, James; Sunderland, Norman
- Abstract
In a study of anther culture in Sorghum bicolor it was found that culture incubation temperature had a significant effect on yields of microspore-derived callus. A temperature of 33° C was superior to 25°, 28° or 37° C. With fresh, unpretreated material, callus was produced only from anthers incubated at 33° C. Panicle pretreatment at 7°, 14°, 20°, 25° or 28° C either had no effect or significantly decreased callus yield. Comparison of culture media containing a range of concentrations of 2,4-dichlorophenoxyacetic acid (0-3.0 mg l) showed that callus yield increased significantly with concentration up to 2.5 mg l and then declined slightly. Although 1174 calluses were produced, subsequent differentiation was poor and only four albino plants were regenerated.
- Publication
Plant Cell, Tissue & Organ Culture, 1986, Vol 6, Issue 1, p15
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/BF00037754