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- Title
Complete genomic sequence of Raphanus sativus cryptic virus 4 (RsCV4), a novel alphapartitivirus from radish.
- Authors
Svanella-Dumas, Laurence; Theil, Sébastien; Barret, Matthieu; Candresse, Thierry
- Abstract
The present work reports the discovery and complete genome sequencing of a virus from symptomless radish seedlings, classifiable as a novel member of the genus <italic>Alphapartitivirus</italic>, family <italic>Partitiviridae</italic>. Total RNA extracted from germinating seedlings was sequenced using Illumina technology. Bioinformatic analysis of the RNA-seq data revealed two contigs representing the near full-length genomic sequences of two genomic RNAs representing a new virus. Analysis of the genome sequence (excluding the polyA tail, RNA1: 1976 nt and RNA2: 1751 nt, respectively) showed a genomic organization typical of viruses classed within the <italic>Partitiviridae</italic>, with each genomic RNA encoding a single open reading frame (ORF). Phylogenetic analysis of the RNA dependent RNA polymerase (RNA1 ORF) and of the capsid protein (RNA2 ORF) clearly showed the new virus can be classified within the genus <italic>Alphapartitivirus</italic>, but sequence divergence establishes it as a new species, for which the name “<italic>Raphanus sativus cryptic virus 4</italic>” is proposed.
- Subjects
RADISH diseases &; pests; VIRAL genomes; RNA sequencing; OPEN reading frames (Genetics); RNA polymerases
- Publication
Archives of Virology, 2018, Vol 163, Issue 4, p1097
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-017-3693-6