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- Title
Characterization of the nuclear import and export signals of pseudorabies virus UL31.
- Authors
Li, Meili; Jiang, Si; Wang, Jinlin; Mo, Chuncong; Zeng, Zhancheng; Yang, Yanjia; Chen, Chunke; Li, Xiaowei; Cui, Wei; Huang, Jinlu; Peng, Tao; Cai, Mingsheng
- Abstract
The pseudorabies virus (PRV) UL31 protein (pUL31) is a homologue of the herpes simplex virus 1 pUL31, which is a multifunctional protein that is important for HSV-1 infection. However, little is known concerning the subcellular localization signal of PRV UL31. Here, by transfection with a series of PRV UL31 deletion mutants fused to an enhanced yellow fluorescent protein (EYFP) gene, a bipartite nuclear localization signal (NLS) and a PY motif NLS of UL31 were identified and mapped to amino acids (aa) 4 to 20 (RRRLLRRKSSAARRKTL) and aa 21 to 34 (TRAARDRYAPYFAY), respectively. Additionally, the predicted nuclear export signal (NES) was shown to be nonfunctional. Taken together, this information opens up new avenues for investigating the biological functions of UL31 during PRV infection.
- Subjects
AUJESZKY'S disease virus; HUMAN herpesvirus 1; DELETION mutation; FLUORESCENT proteins; AMINO acids; VIRAL gene mapping; VIRUSES
- Publication
Archives of Virology, 2015, Vol 160, Issue 10, p2591
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-015-2527-7