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- Title
Phenotypes of the ovarian follicular basal lamina predict developmental competence of oocytes.
- Authors
Helen F. Irving-Rodgers; Stephanie Morris; Rachael A. Collett; Teija T. Peura; Margaret Davy; Jeremy G. Thompson; Helen D. Mason; Raymond J. Rodgers
- Abstract
: BACKGROUND The ovarian follicular basal lamina underlies the epithelial membrana granulosa and maintains the avascular intra-follicular compartment. Additional layers of basal lamina occur in a number of pathologies, including pili annulati and diabetes. We previously found additional layers of follicular basal lamina in a significant percentage of healthy bovine follicles. We wished to determine if this phenomenon existed in humans, and if it was related to oocyte function in the bovine. : METHODS AND RESULTS We examined follicles from human ovaries (n = 18) by electron microscopy and found that many follicles had additional layers of basal lamina. Oocytes (n = 222) from bovine follicles with normal or unusual basal laminas were isolated and their ability to undergo in vitro maturation, fertilization and culture to blastocyst was compared. Healthy bovine follicles with a single layer of basal lamina had oocytes with significantly (P < 0.01) greater developmental competence than healthy follicles with additional layers of follicular basal lamina (65% versus 28%). : CONCLUSIONS These findings provide direct evidence that the phenotype of the follicular basal lamina is related to oocyte competence.
- Subjects
PHENOTYPES; BASAL lamina; OVARIAN follicle; OVUM; FERTILIZATION (Biology); BLASTOCYST; HUMAN embryology
- Publication
Human Reproduction, 2009, Vol 24, Issue 4, p936
- ISSN
0268-1161
- Publication type
Article