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- Title
Effect of Chronic Radiation Exposure on Human MicroRNA Expression.
- Authors
Yanishevskaya, M. A.; Blinova, E. A.; Akleyev, A. V.
- Abstract
An estimate of relative expression of mature microRNAs assessed in peripheral blood cells in persons chronically exposed to low-dose radiation in the long-term period was performed. The study included people exposed in the 1950s on the Techa River (Southern Urals, Russia). The cumulative red bone marrow doses (RBM) of the persons in the main study group (33 persons) ranged from 77.7 to 2869.8 mGy (mean value of 698.5 mGy). The comparison group consisted of 30 people with RBM dose not exceeding 70 mGy over their lifetime. Reverse transcription of RNA samples was performed using specific stem-loop primers ("stem-loop"). Changes in the relative content of microRNAs were assessed by real-time PCR on the CFX96 detection system (BioRad, United States). Statistical analysis of the results was performed using the Mann–Whitney U criterion. A significant increase in hsa-miR-125b, hsa-miR-181a, and hsa-miR-16-5p content was detected in exposed people after 60 years, and their expression was found to depend on the RBM dose.
- Subjects
UNITED States; HAIRPIN (Genetics); MICRORNA; BLOOD cells; BONE marrow; RNA; RADIATION exposure
- Publication
Russian Journal of Genetics, 2023, Vol 59, Issue 10, p1050
- ISSN
1022-7954
- Publication type
Article
- DOI
10.1134/S1022795423100150