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- Title
Diseño eficiente de medios para la producción de lacasa, manganeso peroxidasa y endoxilanasa de Trametes versicolor cultivado sobre residuos agroindustriales, mediante modelamiento matemático.
- Authors
Montoya Barreto, Sandra; Patiño Marín, Yesica Alejandra; Sánchez Toro, Óscar Julián
- Abstract
Objective: To investigate the influence of Mn+2 and Cu+2 ions in eight formulations of different solid media (F1-F8) for the production of laccase (LAC) and manganese peroxidase (MnP) with Trametes versicolor. Methodology: The media were formulated with lignocellulosic residues, using two levels of calcium carbonate, manganese sulfate (II) and copper sulfate (II). The formulations were inoculated at 4% (w/w) with T. versicolor PSUWC 430 and incubated for 17 days; concentrations of biomass and reducing sugars, and hemicellulose and lignin degradation were evaluated. A four-way multifactorial experimental design was applied and the activity of LAC, MnP and ENX was determined. Results: The best LAC enzymatic titres were obtained for F5 and F8 with activities of 11.96 U/g dry solid (ss) and 8.36 U/g ss; for MnP, F8 medium showed the highest enzymatic activity (1.32 U/g ss). The maximum ENX activity was 2.63 U/g ss for F1. The highest degradation of lignin (42.5%) and hemicellulose (32.5%) was achieved with F7 medium. A mathematical model for the production of fungal biomass, LAC and MnP activities, and substrate consumption was proposed. Conclusions: The addition of 0.16% of CuSO4 and 0.1% of MnSO4 favored the production of LAC and MnP by Trametes versicolor. The selected operating conditions enabled to obtain a greater synthesis of LAC and ENX. The mathematical model proposed appropriately described the solid-state fermentation process using Trametes versicolor.
- Subjects
MANGANESE peroxidase; TRAMETES versicolor; MANGANOUS sulfate; SOLID-state fermentation; COPPER sulfate; CALCIUM carbonate; HEMICELLULOSE
- Publication
Investigación e Innovación en Ingenierías, 2020, Vol 8, Issue 2, p106
- ISSN
2344-8652
- Publication type
Article
- DOI
10.17081/invinno.8.2.3601