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- Title
Ethyl Acetate Fraction of Bixa orellana and Its Component Ellagic Acid Exert Antibacterial and Anti-Inflammatory Properties against Mycobacterium abscessus subsp. massiliense.
- Authors
Moraes-Neto, Roberval Nascimento; Coutinho, Gabrielle Guedes; Ataíde, Ana Caroline Santos; de Oliveira Rezende, Aline; Nascimento, Camila Evangelista Carnib; de Albuquerque, Rafaela Pontes; da Rocha, Cláudia Quintino; Rêgo, Adriana Sousa; de Sousa Cartágenes, Maria do Socorro; Abreu-Silva, Ana Lúcia; dos Santos, Igor Victor Ferreira; dos Santos, Cleydson Breno Rodrigues; Guerra, Rosane Nassar Meireles; Ribeiro, Rachel Melo; Monteiro-Neto, Valério; de Sousa, Eduardo Martins; Carvalho, Rafael Cardoso
- Abstract
Mycobacterium abscessus subsp. massiliense (Mabs) causes chronic infections, which has led to the need for new antimycobacterial agents. In this study, we investigated the antimycobacterial and anti-inflammatory activities of the ethyl acetate fraction of Bixa orellana leaves (BoEA) and ellagic acid (ElAc). In silico analysis predicted that ElAc had low toxicity, was not mutagenic or carcinogenic, and had antimicrobial and anti-inflammatory activities. Apparently, ElAc can interact with COX2 and Dihydrofolate reductase (DHFR) enzymes, which could explain both activities. In vitro analysis showed that BoEA and ElAc exerted antimicrobial activity against Mabs (minimum inhibitory concentration of 1.56, 1.56 mg/mL and bactericidal concentration of 6.25, 3.12 mg/mL, respectively. Clarithromycin showed MIC and MBC of 1 and 6 µg/mL). Treatment with BoEA or ElAc increased survival of Tenebrio molitor larvae after lethal infection with Mabs and reduced carrageenan-induced paw edema in mice, around 40% of edema volume after the fourth hour, similarly to diclofenac. In conclusion, BoEA and ElAc exert antimicrobial effects against Mabs and have anti-inflammatory effects, making them potential sources of antimycobacterial drugs. The biological activities of ElAc may be due to its high binding affinities predicted for COX2 and DHFR enzymes.
- Subjects
ELLAGIC acid; ETHYL acetate; MYCOBACTERIUM; TENEBRIO molitor; TETRAHYDROFOLATE dehydrogenase
- Publication
Antibiotics (2079-6382), 2022, Vol 11, Issue 6, p817
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics11060817