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- Title
Detection of Antimicrobial Resistance of Bacteria Staphylococcus chromogenes Isolated from Sheep's Milk and Cheese.
- Authors
Regecová, Ivana; Výrostková, Jana; Zigo, František; Gregová, Gabriela; Kováčová, Mariana; Barlow, John; Martínez, Laura Selva
- Abstract
Antimicrobial and multidrug resistance is detected in nonaureus staphylococci, including Staphylococcus chromogenes, which commonly causes intramammary infections. Recent clinical studies point to the presence of methicillin-resistant S. chromogenes. Therefore, this study aims to determine the prevalence of this species in samples of sheep's milk and cheeses made from them. Isolates were identified by polymerase chain reaction and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF). A total of 208 staphylococcal isolates were identified. Of these, 18% were identified as S. chromogenes. The antimicrobial resistance of the identified isolates was determined using the agar dilution method against penicillin, ceftaroline, teicoplanin, gentamicin, erythromycin, tetracycline, and ofloxacin. The highest resistance was found to penicillin (95%), tetracycline (86%), and oxacillin (81%). The highest sensitivity was confirmed for gentamicin (55%). The study also confirmed the presence of methicillin resistant staphylococcal isolates (30%) based on the phenotypic manifestation of antimicrobial resistance and detection of the presence of the mecA gene. The study shows that the tested isolates (62%) were multidrug resistant. Resistance to two antibiotics was most often found (39%).
- Subjects
SHEEP milk; DRUG resistance in microorganisms; MICROCOCCACEAE; TIME-of-flight mass spectrometry; ERYTHROMYCIN; GRAM-positive bacteria; CHEESE
- Publication
Antibiotics (2079-6382), 2021, Vol 10, Issue 5, p570
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics10050570