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- Title
Cell-Penetrating Peptides-Based Liposomal Delivery System Enhanced Immunogenicity of Peptide-Based Vaccine against Group A Streptococcus.
- Authors
Yang, Jieru; Firdaus, Farrhana; Azuar, Armira; Khalil, Zeinab G.; Marasini, Nirmal; Capon, Robert J.; Hussein, Waleed M.; Toth, Istvan; Skwarczynski, Mariusz; Rothan, Hussin
- Abstract
Peptide-based vaccine development represents a highly promising strategy for preventing Group A Streptococcus (GAS) infection. However, these vaccines need to be administered with the help of a delivery system and/or immune adjuvant. Cell-penetrating peptides (CPPs) have been used as a powerful tool for delivering various therapeutic agents, including peptides, as they can overcome the permeability barrier of cell membranes. Here, we used CPPs to deliver our lead lipopeptide-based vaccine (LCP-1). CPPs were anchored through a spacer to LCP-1-bearing multilamellar and unilamellar liposomes and administered to Swiss outbred mice. Tat47–57 conjugated to two palmitic acids via a (Gly)6 spacer (to form a liposome-anchoring moiety) was the most efficient system for triggering immune responses when combined with multilamellar liposomes bearing LCP-1. The immunostimulatory potential of a variety of other CPPs was examined following intranasal administration in mice. Among them, LCP-1/liposomes/Tat47–57 and LCP-1/liposomes/KALA induced the highest antibody titers. The antibodies produced showed high opsonic activity against clinically isolated GAS strains D3840 and GC2 203. The use of the CPP-liposome delivery system is a promising strategy for liposome-based GAS vaccine development.
- Subjects
INTRANASAL administration; STREPTOCOCCUS; CELL-penetrating peptides; IMMUNOLOGICAL adjuvants; LABORATORY mice; ANTIGENS
- Publication
Vaccines, 2021, Vol 9, Issue 5, p499
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines9050499