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- Title
Crystal structures of FolM alternative dihydrofolate reductase 1 from Brucella suis and Brucella canis.
- Authors
Porter, Imani; Neal, Trinity; Walker, Zion; Hayes, Dylan; Fowler, Kayla; Billups, Nyah; Rhoades, Anais; Smith, Christian; Smith, Kaelyn; Staker, Bart L.; Dranow, David M.; Mayclin, Stephen J.; Subramanian, Sandhya; Edwards, Thomas E.; Myler, Peter J.; Asojo, Oluwatoyin A.
- Abstract
Members of the bacterial genus Brucella cause brucellosis, a zoonotic disease that affects both livestock and wildlife. Brucella are category B infectious agents that can be aerosolized for biological warfare. As part of the structural genomics studies at the Seattle Structural Genomics Center for Infectious Disease (SSGCID), FolM alternative dihydrofolate reductases 1 from Brucella suis and Brucella canis were produced and their structures are reported. The enzymes share ∼95% sequence identity but have less than 33% sequence identity to other homologues with known structure. The structures are prototypical NADPH‐dependent short‐chain reductases that share their highest tertiary‐structural similarity with protozoan pteridine reductases, which are being investigated for rational therapeutic development.
- Subjects
TETRAHYDROFOLATE dehydrogenase; BRUCELLA; CANIS; CRYSTAL structure; BIOLOGICAL warfare; ZOONOSES
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2022, Vol 78, Issue 1, p31
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X21013078